Laboratory analysis

• Author: Don Bartell/Mary Catherine McMurray/Anne ImObersteg
• Pages: 419-446

16-1

CHAPTER 16

LABORATORY ANALYSIS

I. DETERMINATION OF THE ALCOHOL CONCENTRATION

§16:01 Available Methods

§16:02 Validation of the Method

II. VALIDATION OF INSTRUMENTS

§16:10 General Points

§16:11 Precision and Accuracy

§16:12 Specif‌icity and Selectivity

§16:13 Linearity

§16:14 Limit of Detection and Limit of Quantitation

§16:15 Revalidation

III. GAS CHROMATOGRAPHY

A. The Basics of Gas Chromatography

§16:20 The Gas Chromatograph

§16:21 Injection and Separation

§16:21.1 Co-Elution -The Problem with Single Column Gas Chromatography

§16:21.2 The American Board of Forensic Toxicology Requires Two Column GC

§16:21.3 Other Sources Are in Agreement with the Need for Dual Column GC

§16:21.4 Dual Column GC/FID Has the Potential for False Positive Ethanol Results

§16:21.5 The Breakthrough Dual Column GC/FID Co-elution Study

§16:22 Detection and Retention Time

§16:23 Preparing the Specimen for Quantitation

§16:24 Calculating the Ethanol Concentration

B. Evaluating the Process

§16:30 The Quality of the Chromatograms

§16:31 Headspace vs. Direct Injection

C. GC Analytical Procedure

§16:40 Preparing the Run

§16:41 Quality Checks in the Run

§16:42 Quality Checks After the Run

D. Discovery

§16:50 Document Review

§16:51 Evaluating the Case Chromatogram

LABORATORY ANALYSIS

Attacking and Defending Drunk Driving Tests 16-2

LABORATORY ANALYSIS

§16:52 Archiving of Raw Data

§16:53 Maintenance and Revalidation

IV. ENZYME IMMUNOASSAY

§16:60 Immunochemical Methods

§16:61 Immunoassay Analysis vs. Gas Chromatography

V. QUALITY CONTROL SOLUTIONS

§16:70 Reference Solutions

§16:71 The Simulator and Simulator Solutions

§16:72 Calibration Checks With Dry Gas

§16:73 Reagents and Controls

VI. RISK OF ERROR

§16:80 Error Is Unavoidable

§16:81 Checklist of Sources of Error

§16:82 Summary